| Development of in-feedlot diagnostics for the accurate and timely identification of pathogens associated with bovine respiratory disease complex (BRDC) presents specific challenges due to the necessary logistics of feedlot practice and animal management. Staff ratios associated with intensive beef production, coupled with the requirement for minimum interventions, means that a point-of-decision diagnostic must be fast, accurate and reliable but also inexpensive to be useful and effective in improving animal outcomes as well as making a meaningful impact on economic margins at induction. Necessary minimisation of pulls for intervention and / or treatment equally presents a challenge for diagnosis and management of BRDC-affected animals in the hospital system, who rarely undergo detailed diagnostic workup. To be an effective strategy for the in-feedlot analysis of the major bacterial pathogens associated with bovine respiratory disease complex, the timeline for sampling and results must fit within existing feedlot workflows to be adopted by industry. A co-design approach was undertaken to develop an in-feedlot diagnostic tool capable of informing BRDC management decisions which will be discussed. This approach is based on quantitative PCR with increased accuracy of the results and will also be highlighted using analysis of induction and hospital pen cattle from two commercial feedlots in Australia.